| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Li, Julia (16)
|
MAWCAY |
58.44 |
56.18 |
-2.26 |
| 2 |
Pryshchenko, Katya A (16)
|
MAMSA |
57.19 |
57.98 |
0.79 |
| 3 |
Kingsley, Emma B (15)
|
MAFSA |
56.78 |
58.85 |
2.07 |
| 4 |
Washlick, Mary R (16)
|
MAGRA |
58.15 |
1:00.32 |
2.17 |
| 5 |
Cieslukowski, Caitlin A (15)
|
MAWCAY |
58.93 |
1:01.35 |
2.42 |
| 6 |
Davis, Josephine W (15)
|
MAUN |
1:02.00 |
1:01.55 |
-0.45 |
| 7 |
Zeminski, Caroline (15)
|
MAFWAC |
1:02.57 |
1:02.12 |
-0.45 |
| 7 |
Bakker, Anna C (15)
|
MAMSA |
1:02.51 |
1:02.12 |
-0.39 |
| 9 |
Green, Riley H (16)
|
MAFWAC |
1:03.80 |
1:02.41 |
-1.39 |
| 10 |
Niemann, Brianna N (16)
|
MAJDEL |
1:01.81 |
1:03.44 |
1.63 |
| 11 |
Nishikawa, Lauren (15)
|
MAACAC |
1:04.56 |
1:04.08 |
-0.48 |
| 12 |
Holm, Fiona C (15)
|
MACAA |
1:05.01 |
1:04.36 |
-0.65 |
| 13 |
Schuster, Ciara E (15)
|
MAWCAY |
1:05.63 |
1:04.81 |
-0.82 |
| 14 |
Krauss, Rachel M (15)
|
MAWCAY |
1:09.91 |
1:06.68 |
-3.23 |
| 15 |
Bossi, Courtney A (16)
|
MACAA |
1:07.93 |
1:09.69 |
1.76 |
| 16 |
Shirey, Talia M (16)
|
MAUN |
1:14.44 |
1:11.08 |
-3.36 |
| 17 |
Wilson, Hannah M (16)
|
MAUN |
|
1:12.19 |
- |
|
Oswald, Abigail C (16)
|
MAMSA |
|
1:08.87 |
- |
|
LaBree, Hunter (16)
|
MANSC |
1:09.49 |
NS |
- |
|
Bluestein, Talia R (15)
|
MANSC |
1:03.46 |
NS |
- |
|
Kaplan, Jordan A (16)
|
MAFWAC |
1:12.83 |
NS |
- |